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Methods of Protein Microcharacterization pp 363–377Cite as

COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methods

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Part of the book series: Biological Methods ((BM))

Abstract

Whereas the complete primary structure of a polypeptide may be established by mass spectrometry (see chapters by Wittmann-Liebold and L’Italien in this volume), this cannot be achieved by Edman degradation because this stepwise procedure does not allow discrimination between polypeptides with free or blocked COOH-terminal carboxylic groups. This shortcoming of Edman degradation must not be ignored in view of the significant biologic role of COOH-terminal modifications, especially for many regulatory pep-tides (1,2). Thus, whenever Edman degradation is used as the prinicpal method of complete amino acid sequence determination, it must be supplemented by COOH-terminal analysis.

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Author information

Authors and Affiliations

  1. The Salk Institute, La Jolla, California

    Joachim Spiess

Authors
  1. Joachim Spiess
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Editor information

Editors and Affiliations

  1. Beckman Research Institute of the City of Hope, Duarte, California

    John E. Shively

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© 1986 The Humana Press Inc.

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Spiess, J. (1986). COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methods. In: Shively, J.E. (eds) Methods of Protein Microcharacterization. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-59259-436-8_14

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  • DOI: https://doi.org/10.1007/978-1-59259-436-8_14

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-090-9

  • Online ISBN: 978-1-59259-436-8

  • eBook Packages: Springer Book Archive

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