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Microsequence Analysis by Enzymatic Methods

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Methods of Protein Microcharacterization

Part of the book series: Biological Methods ((BM))

Abstract

The amino-terminal sequence analysis of polypeptides by the Edman procedure has become a standard and routine method for the protein chemist. The procedure combines the specificity of end-group analysis with the advantages of a cyclic stepwise process. This powerful technique has been automated and normally permits the unambiguous identification of the first 30–50 residues of a polypeptide. Recent modifications of the automated Edman procedure now permit the microsequence analysis of proteins (see chapter by Hunkapiller in this volume). This important development makes possible the structure analysis of proteins at the picomole level. Thus, many protein structural studies are now being undertaken that would have been impossible a few years ago.

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Author information

Authors and Affiliations

  1. Unigene Laboratories, Inc., Fairfield, New Jersey

    Barry Nelson Jones

Authors
  1. Barry Nelson Jones
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Editor information

Editors and Affiliations

  1. Beckman Research Institute of the City of Hope, Duarte, California

    John E. Shively

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© 1986 The Humana Press Inc.

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Jones, B.N. (1986). Microsequence Analysis by Enzymatic Methods. In: Shively, J.E. (eds) Methods of Protein Microcharacterization. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-59259-436-8_13

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  • DOI: https://doi.org/10.1007/978-1-59259-436-8_13

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-090-9

  • Online ISBN: 978-1-59259-436-8

  • eBook Packages: Springer Book Archive

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