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Nonisotopic In Situ Hybridization

Gene Mapping and Cytogenetics

  • Protocol
Immunochemical Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 80))

Abstract

With the advent of nonradioactive probes during the past decade, in situ hybridization has become an immensely valuable tool in the hands of:

  1. 1.

    Developmental biologists and cell biologists for the detection of mRNA in cells and tissue sections (1–7),

  2. 2.

    Virologists for detection of viral DNA and RNA (8), and

  3. 3.

    Molecular cytogeneticists in detecting chromosome aberrations in interphase cells (Interphase Cytogenetics, especially Cancer Genetics, 9–11), sex determination (12,13), and gene mapping by nonisotopic in situ hybridization (NISH, 14,15).

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Author information

Authors and Affiliations

  1. Bone and Joint Research Unit, London Hospital Medical College, London, UK

    Bhupendra Bhatt, Tulin Sahinoglu & Cliff Stevens

Authors
  1. Bhupendra Bhatt
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  2. Tulin Sahinoglu
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  3. Cliff Stevens
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Editor information

Editors and Affiliations

  1. The University of Birmingham, UK

    John D. Pound

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© 1998 Humana Press Inc., Totowa, NJ

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Bhatt, B., Sahinoglu, T., Stevens, C. (1998). Nonisotopic In Situ Hybridization. In: Pound, J.D. (eds) Immunochemical Protocols. Methods in Molecular Biology™, vol 80. Humana Press. https://doi.org/10.1007/978-1-59259-257-9_41

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  • DOI: https://doi.org/10.1007/978-1-59259-257-9_41

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-493-8

  • Online ISBN: 978-1-59259-257-9

  • eBook Packages: Springer Protocols

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