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Immunochemical Protocols pp 283–295Cite as

Immunogold Probes in Light Microscopy

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 80))

Abstract

All immunocytochemical techniques are based on the same principle of incubating the target antigen with an appropriate antibody solution, but they may incorporate one from a range of different microscopically dense markers for visualizing, the sites of binding. Light microscope immunocytochemistry was initiated by the classical work of Coons and coworkers in 1950, who developed the immunofluorescence technique for antigen localization (1,2). This was followed some time later in 1966 with the introduction by Nakane and Pierce of the lmmunoperoxidase procedure (3), after which there have been various attempts to increase the sensitivity of techniques for localizing tissue antigens, including peroxidase antiperoxidase (PAP) (4), avidin-biotin complex (5), and alkaline phosphatase antialkaline phosphatase (APAAP) (6). These techniques are all still routinely used for immunocytochemistry.

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Author information

Authors and Affiliations

  1. Nuffield Department of Surgery, John Radcliffe Hospital, Oxford, UK

    David A. Hughes

  2. Department of Molecular Pathology, Glaxo-Wellcome Medicines Research Centre, Stevenage, UK

    Julian E. Beesley

Authors
  1. David A. Hughes
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  2. Julian E. Beesley
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Editor information

Editors and Affiliations

  1. The University of Birmingham, UK

    John D. Pound

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© 1998 Humana Press Inc., Totowa, NJ

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Hughes, D.A., Beesley, J.E. (1998). Immunogold Probes in Light Microscopy. In: Pound, J.D. (eds) Immunochemical Protocols. Methods in Molecular Biology™, vol 80. Humana Press. https://doi.org/10.1007/978-1-59259-257-9_30

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  • DOI: https://doi.org/10.1007/978-1-59259-257-9_30

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-493-8

  • Online ISBN: 978-1-59259-257-9

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