Abstract
Cell kinetics is defined as the measurement of time parameters in biological systems. Traditionally, this has involved the use of radioactive precursors of DNA, such as tritiated thymidine (3HTdR), and autoradiography to detect their incorporation into DNA. This technique has provided detailed knowledge of cell kinetics in both in vitro and in vivo experimental systems. The technique, however, is time consuming and arduous and is not readily applicable to human tumor research because of ethical problems involved in incorporation of a radioisotope into DNA.
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References
Gratzner, H (1982) Monoclonal antibody against 5-bromo and 5-iodo-deoxyuridine: a new reagent for detection of DNA replication. Science 218, 474,475
Dolbeare, F., Gratzner, H, Pallavicini, M, and Gray, J W (1983) Flow cytometric measurement of total DNA content and incorporated bromodeoxyundine Proc Natl Acad Sci USA 80, 5573–5577
Pallavicini, M. G (1987) Solid tissue dispersal for cytokinetic analyses, in Techniques in Cell Cycle Analysis (Gray, J. W and Darzynewicz, Z., eds.), Humana, Clifton, NJ, pp. 139–162.
Wilson, G D, McNally, N. J, Dunphy, E, Karcher, H., and Pfragner, R (1985) The labelling index of human and mouse tumours assessed by bromo-deoxyuridine staining in vitro and in vivo and flow cytometry Cytometry 6, 641–647.
Begg, A. C, McNally, N J., Shrieve, D. C, and Karcher, H. (1985) A method to measure the duration of DNA synthesis and the potential doubling from a single sample. Cytometry 6, 620–626.
Wilson, G. D, McNally, N. J., Dische, S., Saunders, M. I., des Rochers, C, Lewis, A. A., and Bennett, M H. (1988) Measurement of cell kinetics in human tumours in vivo using bromodeoxyuridine incorporation and flow cytometry. Br J Cancer 58, 423–431.
Schutte, B, Reynders, M. M. J., van Assche, C. L. M. V. J., Hupperets, P. S. G. J, Bosman, F. T, and Blijham, G. H. (1987) An improved method for the immuno-cytochemical detection of bromodeoxyuridine labelled nuclei using flow cytometry Cytometry 8, 372–376
Beisker, W., Dolbeare, F., and Gray, J W. (1987) An improved lmmunocytochemical procedure for high sensitivity detection of incorporated bromodeoxyuridine. Cytometry 8, 235–239
van Erp, P E J, Brons, PPT, Boezeman, J. B M., de Jongh, G J, and Bauer, F. W. (1988) A rapid flow cytometric method for bivariate bromodeoxyuridine/DNA analysis using simultaneous proteolytic enzyme digestion and acid denaturation. Cytometry 9, 627–630.
Dolbeare, F. and Gray, J. W. (1988) Use of restriction endonuclease and exonuclease III to expose halogenated pyrimidines for immunochemical staining Cytometry 9, 631–635
Dolbeare, F, Kuo, W. L., Vanderlaan, M., and Gray, J W (1988) Cell cycle analysis by flow cytometric analysis of the incorporation of lododeoxyuridine (IdUrd) and bromodeoxyuridine (BrdUrd) Proc Am Assoc Cancer Res 29, 1896–1901
Vanderlaan, M, Watkins, B, Thomas, C, Dolbeare, F, and Stanker, L. (1986) Improved high-affinity monoclonal antibody to iododeoxyuridine Cytometry 7, 499–507.
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© 1998 Humana Press Inc., Totowa, NJ
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Wilson, G.D. (1998). Cell Kinetic Studies Using a Monoclonal Antibody to Bromodeoxyuridine. In: Pound, J.D. (eds) Immunochemical Protocols. Methods in Molecular Biology™, vol 80. Humana Press. https://doi.org/10.1007/978-1-59259-257-9_26
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DOI: https://doi.org/10.1007/978-1-59259-257-9_26
Publisher Name: Humana Press
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Online ISBN: 978-1-59259-257-9
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