Skip to main content
SpringerLink
Log in
Book cover

Plant and Food Carotenoids pp 375–386Cite as

In Vitro Digestion for Control and Monitoring of Food Effects in Relation to Micellarization Index of Carotenoids

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2083))

Abstract

The multifactorial system that influences the availability of macronutrients, micronutrients, and phytoconstituents with relevant bioactivities has been assessed by in vitro digestion protocols, which have become an effective technique to support the health-through-food strategy. The measurement of the significance of food structure, food matrix, synergies and competitive effects, processing features, and even some physiological issues has created valuable scientific and technological information, and the development of harmonized protocols. Now it is possible to make further advances by applying this knowledge to obtain data regarding the potential availability of target compound(s) in the food source within a standard meal context. This protocol describes the measurement of the micellarization index of carotenoids from dietary rich sources with test meals accounting the effects of high vs. low fat and normo-, hypo-, and hypercaloric content.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Arranz E, Corredig M, Guri A (2016) Designing food delivery systems: challenges related to the in vitro methods employed to determine the fate of bioactives in the gut. Food Funct 7:3319–3336

    Article  CAS  Google Scholar 

  2. Minekus M, Alminger M, Alvito P, Balance S, Bohn T, Bourlieu C, Carriere F, Boutrou R, Corredig M, Dupont D, Dufour C, Egger L, Golding M, Karakaya S, Kirkhus B, Le Feunteun S, Lesmes U, Macierzanka A, Mackie A, Marze S, McClements DJ, Ménard O, Recio I, Santos CN, Singh RP, Vegarud GE, Wickham MSJ, Weitschies W, Brodkorb A (2014) A standardized static in vitro digestion method suitable for food—an international consensus. Food Funct 5:113–1124

    Google Scholar 

  3. Bohn T, McDougall GJ, Alegría A, Alminger M, Arrigoni E, Aura AM, Brito C, Cilla A, El SN, Karakaya S, Martínez-Cuesta MC, Santos CN (2015) Mind the gap-deficits around our knowledge on aspects impacting bioavailability of phytochemicals and their metabolites. Mol Nutr Food Res 59:1307–1323

    Article  CAS  Google Scholar 

  4. Desmarcherlier C, Borel P (2017) Overview of carotenoid bioavailability determinants: from dietary factors to host genetic variations. Trends Food Sci Nutr 69:270–280

    Article  Google Scholar 

  5. Levi CS, Alvito P, Andrés A, Assuncao R, Barberá R, Blanquet-Diot S, Bourlieu C, Brodkorb A, Cilla A, Deglaire A, Denis S, Dupont D, Heredia A, Karakaya S, Giosafatto CVL, Mariniello L, Martins C, Ménard O, El SN, Vegarud GE, Ulleberg E, Lesmes U (2017) Extending in vitro digestion models to specific human populations: perspectives, practical tools and bio-relevant information. Trends Food Sci Technol 60:52–63

    Article  Google Scholar 

  6. de Oliveira SC, Deglaire AM, Ménard O, Bellanger A, Rousseau F (2016) Holder pasteurization impacts the proteolysis, lipolysis and disintegration of human milk under in vitro dynamic term newborn digestion. Food Res Int 88:263–275

    Article  Google Scholar 

  7. Garrett DA, Failla ML, Sarama RJ (1999) Development of an in vitro digestion method to assess carotenoid bioavailability from meals. J Agric Food Chem 47:4301–4309

    Article  CAS  Google Scholar 

  8. Reboul E, Richelle M, Perrot E, Desmoulins-Malezet C, Pirisi V, Borel P (2006) Bioaccessibility of carotenoids and vitamin E from their main dietary sources. J Agric Food Chem 54:8749–8755

    Article  CAS  Google Scholar 

  9. Pérez-Gálvez A (2019) In vitro digestion protocols. The benchmark for estimation of in vivo data. In: Mercadante A (ed) Carotenoid esters in foods. Royal Society of Chemistry, London. (in press)

    Google Scholar 

  10. U.S. Food and Drug Administration, Center for Drug Evaluation and Research (2002). Guidance for industry: food-effect bioavailability and fed bioequivalence studies. Office of Training and Communications, Division of Drug Information HFD-240 Rockville Maryland 20857

    Google Scholar 

  11. Food and agriculture organization of the United Nations/World Health Organization/United Nations University (2011) Human energy requirements

    Google Scholar 

  12. Joint WHO/FAO Expert Consultation on Diet, Nutrition and the Prevention of Chronic Diseases (2002). Diet, Nutrition and the prevention of chronic diseases: report of a joint WHO/FAO expert consultation WHO technical report series 916

    Google Scholar 

  13. Mataix-Verdú J (ed) (2011) Tabla de composición de alimentos. Granada, EUG

    Google Scholar 

  14. O’Neill ME, Carroll Y, Corridan B, Olmedilla B, Granado F, Blanco I, van den Berg H, Hiniger I, Rousell AM, Chopra M, Southon S, Thurnham DI (2001) A European carotenoid database to assess carotenoid intakes and its use in a five-country comparative study. Br J Nutr 85:499–507

    Google Scholar 

  15. Ríos JJ, Xavier AAO, Díaz-Salido E, Arenilla-Vélez I, Jarén-Galán M, Garrido-Fernández J, Aguayo-Maldonado J, Pérez-Gálvez A (2017) Xanthophyll esters are found in human colostrum. Mol Nutr Food Res 61:1700296

    Article  Google Scholar 

  16. Britton G, Liaaen-Jensen S, Pfander H (eds) (1995) carotenoids. Volume 1A: isolation and analysis. Birkhäuser Verlag. Baseline

    Google Scholar 

  17. Pérez-Gálvez A, Sánchez-García A, Garrido-Fernández J, Ríos JJ (2018) MS tools for a systematic approach in survey for carotenoids and their common metabolites. Arch Biochem Biophys 650:85–92

    Article  Google Scholar 

  18. Burger W, Burger MJ (eds) (2010) Digital image processing: an algorithmic introduction using java. Springer, Berlin

    Google Scholar 

Download references

Acknowledgments

The financial support of the Ministerio de Economía y Competitividad (MINECO-CICYT, Spanish Government, project AGL2017-87884-R) is gratefully acknowledged. Antonio Pérez-Gálvez is a tenured scientist at the Spanish National Research Council (CSIC).

Author information

Authors and Affiliations

  1. Food Phytochemistry Department, Instituto de la Grasa (CSIC), Sevilla, Spain

    Antonio Pérez-Gálvez

  2. Institute of Food Science Research (CSIC-UAM), Madrid, Spain

    Javier Fontecha

Authors
  1. Antonio Pérez-Gálvez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Javier Fontecha
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Antonio Pérez-Gálvez .

Editor information

Editors and Affiliations

  1. CSIC-IRTA-UAB-UB, Centre for Research in Agricultural Genomics (CRAG), Barcelona, Spain

    Manuel Rodríguez-Concepción

  2. Institute for Biology II, University of Freiburg, Freiburg, Germany

    Ralf Welsch

Rights and permissions

Reprints and permissions

Copyright information

© 2020 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Pérez-Gálvez, A., Fontecha, J. (2020). In Vitro Digestion for Control and Monitoring of Food Effects in Relation to Micellarization Index of Carotenoids. In: Rodríguez-Concepción, M., Welsch, R. (eds) Plant and Food Carotenoids. Methods in Molecular Biology, vol 2083. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9952-1_28

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-9952-1_28

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9951-4

  • Online ISBN: 978-1-4939-9952-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation