Abstract
In vivo bioluminescent imaging allows the detection of reporter gene expression in rodents in real time. Here we describe a novel technology whereby we can generate somatotransgenic rodents with the use of a viral vector carrying a luciferase transgene. We are able to achieve long term luciferase expression by a single injection of lentiviral or adeno-associated virus vectors to newborn mice. Further, we describe whole body bioluminescence imaging of conscious mice in a noninvasive manner, thus enforcing the 3R’s (replacement, reduction, and refinement) of biomedical animal research.
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Acknowledgments
J.M.K.M.D. and S.N.W. were funded by the ERC grant Somabio (260862), T.R.M. and S.N.W. were funded by the NC3Rs (NC/L001780/1). R.K. and S.N.W. received funding from MRC grants MR/P026494/1 and MR/R015325/1, and from SPARKS grant 17UCL01. J.A.D. is funded by CONICYT Becas Chile Doctoral Fellowship Program 72160294.
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Diaz, J.A. et al. (2020). Continual Conscious Bioluminescent Imaging in Freely Moving Mice. In: Ripp, S. (eds) Bioluminescent Imaging. Methods in Molecular Biology, vol 2081. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9940-8_11
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DOI: https://doi.org/10.1007/978-1-4939-9940-8_11
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