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Macrophage Migration Inhibitory Factor pp 147–158Cite as

Inducing and Inhibiting Autophagy to Investigate Its Interactions with MIF

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2080))

Abstract

MIF has been described to be associated with autophagy in a number of studies, but the full nature of their association is not yet clear. However, the unprecedented interest in autophagy in recent times has generated a number of tools and techniques for its study. Here, we present protocols for studying the interactions between MIF and autophagy, including for the induction and inhibition of autophagy and measuring autophagosome biogenesis and maturation.

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Acknowledgements

We would like to thank Jurie Tashkandi for assistance with the experiment shown in Fig. 1.

Author information

Authors and Affiliations

  1. The Ritchie Centre, Hudson Institute of Medical Research, Clayton, VIC, Australia

    Nadia S. Deen

  2. Department of Medicine, University of Cambridge, Cambridge, UK

    Jacinta P. Lee

  3. Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical Centre, Monash University, Clayton, VIC, Australia

    James Harris

Authors
  1. Nadia S. Deen
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  2. Jacinta P. Lee
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  3. James Harris
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Corresponding author

Correspondence to James Harris .

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Editors and Affiliations

  1. Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical Centre, Monash University, Clayton, VIC, Australia

    James Harris

  2. Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical Centre, Monash University, Clayton, VIC, Australia

    Eric F. Morand

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Deen, N.S., Lee, J.P., Harris, J. (2020). Inducing and Inhibiting Autophagy to Investigate Its Interactions with MIF. In: Harris, J., Morand, E. (eds) Macrophage Migration Inhibitory Factor. Methods in Molecular Biology, vol 2080. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9936-1_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9936-1_13

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9935-4

  • Online ISBN: 978-1-4939-9936-1

  • eBook Packages: Springer Protocols

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