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Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies

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Antibody-Drug Conjugates

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2078))

Abstract

Antibodies bearing engineered cysteine residues (termed THIOMAB™ antibodies) enable the site-selective attachment of a drug, label or other payload for specific delivery to certain tissues (e.g., tumors). This Chapter describes detailed methods we have developed and optimized for the conjugation, purification and analysis of THIOMAB™ antibody drug conjugates (TDCs).

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References

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Acknowledgements

We acknowledge Martine Darwish, Breanna Vollmar, and Richard Vandlen for helpful comments.

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Authors and Affiliations

  1. Department of Protein Chemistry, Genentech, Inc., South San Francisco, CA, USA

    Pragya Adhikari, Neelie Zacharias, Rachana Ohri & Jack Sadowsky

Authors
  1. Pragya Adhikari
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  2. Neelie Zacharias
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  3. Rachana Ohri
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  4. Jack Sadowsky
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Corresponding author

Correspondence to Jack Sadowsky .

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Editors and Affiliations

  1. Department of Pharmacy and Pharmaceutical Sciences, Binghamton University, Binghamton, NY, USA

    L. Nathan Tumey

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Adhikari, P., Zacharias, N., Ohri, R., Sadowsky, J. (2020). Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies. In: Tumey, L. (eds) Antibody-Drug Conjugates. Methods in Molecular Biology, vol 2078. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9929-3_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9929-3_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9928-6

  • Online ISBN: 978-1-4939-9929-3

  • eBook Packages: Springer Protocols

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