Abstract
Cloning the open reading frame (ORF) of a protein-coding chimeric RNA into a mammalian expression vector is a simple, practiced way to study the function of the chimeric RNA. Here, we provide procedures for the insertion of the RRM2-C2orf48 fusion ORF into a mammalian expression vector, achieving the overexpression of the RRM2-C2orf48 fusion protein in a colon cancer cell line by retroviral transduction.
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Wu, H., Li, H. (2020). Overexpression of Chimeric RNA by Retroviral Transduction. In: Li, H., Elfman, J. (eds) Chimeric RNA. Methods in Molecular Biology, vol 2079. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9904-0_12
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DOI: https://doi.org/10.1007/978-1-4939-9904-0_12
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Online ISBN: 978-1-4939-9904-0
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