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Methods to Identify and Analyze Vesicle-Protected DNA Transfer

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Horizontal Gene Transfer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2075))

Abstract

Conjugation, transformation, and transduction constitute the three classical mechanisms involved in horizontal gene transfer (HGT) among prokaryotes. In addition, alternative HGT mechanisms exist in groups of organisms. Among them, the use of DNA-containing membrane vesicles as shuttle elements for HGT has been described for a number of microorganisms, including both thermophiles and mesophiles. Here we describe the methods followed to detect, purify, and analyze these vesicles.

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Acknowledgments

This work has been supported by grant BIO2016-77031-R from the Spanish Ministry of Economy and Competitiveness and grant number 324439 of the FP7-PEOPLE-2012-IAPP from the European Union. An institutional grant from Fundación Ramón Areces to CBMSO is also acknowledged. Protein identification by LC/MS/MS was carried out in the “CBMSO PROTEIN CHEMISTRY FACILITY” that belongs to ProteoRed, PRB2-ISCIII, supported by grant PT13/0001. Technical assistance of E. Sánchez is also acknowledged.

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Authors and Affiliations

  1. Centro de BiologÚa Molecular Severo Ochoa, Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas, Madrid, Spain

    Alba Blesa & José Berenguer

  2. Department of Biotechnology, Faculty of Experimental Sciences, Universidad Francisco de Vitoria, Madrid, Spain

    Alba Blesa

Authors
  1. Alba Blesa
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  2. José Berenguer
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Corresponding author

Correspondence to José Berenguer .

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Editors and Affiliations

  1. Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria-CSIC, Santander, Cantabria, Spain

    Fernando de la Cruz

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Blesa, A., Berenguer, J. (2020). Methods to Identify and Analyze Vesicle-Protected DNA Transfer. In: de la Cruz, F. (eds) Horizontal Gene Transfer. Methods in Molecular Biology, vol 2075. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9877-7_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9877-7_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9876-0

  • Online ISBN: 978-1-4939-9877-7

  • eBook Packages: Springer Protocols

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