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Protein Nanotechnology pp 247–285Cite as

Atomic Force Microscopy of Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2073))

Abstract

Atomic force microscopy (AFM) enables imaging of surface-deposited proteins and protein structures under physiological conditions, which is a benefit compared to ultra-high vacuum techniques such as electron microscopy. AFM also has the potential to provide more information from the phase in tapping mode or from functional AFM modes. The sample preparation, probe selection, and imaging conditions are crucial for successful imaging of proteins. Here we give a detailed account of the steps toward imaging of soft samples in both air and liquid along with the basic theory underpinning these details.

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Author information

Authors and Affiliations

  1. School of Chemical Sciences, University of Auckland, Auckland, New Zealand

    Yiran An & Sesha Sarathchandra Manuguri

  2. Department of Chemical and Materials Engineering, University of Auckland, Auckland, New Zealand

    Yiran An, Sesha Sarathchandra Manuguri & Jenny Malmström

  3. MacDiarmid Institute for Advanced Materials and Nanotechnology, Auckland, New Zealand

    Sesha Sarathchandra Manuguri & Jenny Malmström

Authors
  1. Yiran An
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  2. Sesha Sarathchandra Manuguri
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  3. Jenny Malmström
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Corresponding author

Correspondence to Jenny Malmström .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, The MacDiarmid Institute for Advanced Materials and Nanotechnology, School of Chemical Sciences, University of Auckland, Auckland, New Zealand

    Juliet A. Gerrard

  2. School of Biological Sciences, Department of Chemical and Materials Engineering, The MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand

    Laura J. Domigan

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An, Y., Manuguri, S.S., Malmström, J. (2020). Atomic Force Microscopy of Proteins. In: Gerrard, J., Domigan, L. (eds) Protein Nanotechnology. Methods in Molecular Biology, vol 2073. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9869-2_14

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  • DOI: https://doi.org/10.1007/978-1-4939-9869-2_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9868-5

  • Online ISBN: 978-1-4939-9869-2

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