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DNA Methylation and Transcriptomic Next-Generation Technologies in Cereal Genomics

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Cereal Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2072))

Abstract

RNA sequencing (RNA-seq) coupled to DNA methylation strategies enables the detection and characterization of genes which expression levels might be mediated by DNA methylation. Here we describe a bioinformatics protocol to analyze gene expression levels using RNA-seq data that allow us to identify candidate genes to be tested by bisulfite assays. The candidate methylated genes are usually those that are low expressed in a particular condition or developmental stage.

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Acknowledgments

The authors work was supported by two grants received from the National Council for Science and Technology (CB2016-285898, CB2016-286368 and INFR-2016-01-269833) and Cátedras Marcos Moshinsky 2017.

Author information

Author notes

  1. Cynthia G. Soto-Cardinault and Fátima Duarte-Aké contributed equally to this work.

Authors and Affiliations

  1. Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico

    Cynthia G. Soto-Cardinault, Fátima Duarte-Aké & Clelia De-la-Peña

  2. CONACYT-Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico

    Elsa Góngora-Castillo

Authors
  1. Cynthia G. Soto-Cardinault
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  2. Fátima Duarte-Aké
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  3. Clelia De-la-Peña
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  4. Elsa Góngora-Castillo
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Corresponding author

Correspondence to Elsa Góngora-Castillo .

Editor information

Editors and Affiliations

  1. Instituto de Diversidad y Ecología Animal, Consejo Nacional de Investigaciones Científicas y Técnicas (IDEA, CONICET), Universidad Nacional de Córdoba, Córdoba, Argentina

    Luis M. Vaschetto

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Soto-Cardinault, C.G., Duarte-Aké, F., De-la-Peña, C., Góngora-Castillo, E. (2020). DNA Methylation and Transcriptomic Next-Generation Technologies in Cereal Genomics. In: Vaschetto, L. (eds) Cereal Genomics. Methods in Molecular Biology, vol 2072. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9865-4_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9865-4_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9864-7

  • Online ISBN: 978-1-4939-9865-4

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