Abstract
Upon fertilization, normal endosperm and embryo development require the contribution of both the maternal and paternal genomes. However, certain genes are expressed in a parent-of-origin-dependent manner, an epigenetic phenomenon known as genomic imprinting. Despite the blast of new technologies and the crucial advances of the past decades in the epigenetics field, novel imprinted genes are yet to be discovered and thus key regulators of early seed development. Using rice plant as a model, we describe a method for the identification of imprinted genes based on an RNA-Seq approach, which allows the identification of maternal and paternal gene expression in a parent-of-origin-specific manner.
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Acknowledgments
This work was supported by a departmental start-up grant from the Environmental Horticulture Department at the University of Florida to K.B.
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Chen, C., Begcy, K. (2020). Genome-Wide Identification of Allele-Specific Gene Expression in a Parent-of-Origin Specific Manner. In: Vaschetto, L. (eds) Cereal Genomics. Methods in Molecular Biology, vol 2072. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9865-4_11
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DOI: https://doi.org/10.1007/978-1-4939-9865-4_11
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