Abstract
Whole-genome bisulfite sequencing (WGBS) is a technique used for the analysis of genome-wide DNA methylation patterns (DNA methylomes) at a single-base resolution. Here, I describe a simple DNA extraction method from rice endosperm and the universal protocol of WGBS, MethylC-sequencing library preparation. The use of benzyl chloride allows for the extraction of high-quality genomic DNA from starchy endosperm, while sodium bisulfite converts unmethylated cytosine to uracil, whereas methylated cytosine is unchanged. The bisulfite conversion of whole genome sequencing libraries before the final amplification step allows for the discrimination of methylated from unmethylated cytosines in a genome-wide manner.
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Acknowledgments
This work is supported by JSPS KAKENHI (17H05851 and 19H04873).
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Kawakatsu, T. (2020). Whole-Genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes. In: Vaschetto, L. (eds) Cereal Genomics. Methods in Molecular Biology, vol 2072. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9865-4_10
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DOI: https://doi.org/10.1007/978-1-4939-9865-4_10
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-4939-9865-4
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