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Assays to Evaluate Toxoplasma–Macrophage Interactions

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Book cover Toxoplasma gondii

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2071))

Abstract

The obligate intracellular protozoan parasite Toxoplasma gondii can infect any nucleated cell from a warm-blooded host. However, its interaction with host macrophages plays a critical role in shaping the immune response during infection. Therefore, assessing Toxoplasma–macrophage interactions at a cellular level is important. In this chapter, we describe assays that can be used to characterize Toxoplasma–macrophage interactions. These assays can also be used to evaluate other host–pathogen interactions. We describe multiplex approaches for measuring arginase activity, indoleamine 2,3 dioxygenase activity, cell death, and parasite growth during Toxoplasma–macrophage interactions. These assays can be used to compare how different Toxoplasma strains differ in their interaction with macrophages, and we describe how to properly assess Toxoplasma strain differences in Toxoplasma–macrophage interactions.

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Author information

Authors and Affiliations

  1. Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, CA, USA

    Debanjan Mukhopadhyay & Jeroen P. J. Saeij

Authors
  1. Debanjan Mukhopadhyay
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  2. Jeroen P. J. Saeij
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Corresponding author

Correspondence to Jeroen P. J. Saeij .

Editor information

Editors and Affiliations

  1. Division of Infectious Disease and Immune Defense, The Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, Australia

    Christopher J. Tonkin

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Mukhopadhyay, D., Saeij, J.P.J. (2020). Assays to Evaluate Toxoplasma–Macrophage Interactions. In: Tonkin, C. (eds) Toxoplasma gondii. Methods in Molecular Biology, vol 2071. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9857-9_19

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  • DOI: https://doi.org/10.1007/978-1-4939-9857-9_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9856-2

  • Online ISBN: 978-1-4939-9857-9

  • eBook Packages: Springer Protocols

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