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Phenotyping Toxoplasma Invasive Skills by Fast Live Cell Imaging

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Toxoplasma gondii

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2071))

Abstract

Host cell invasion by Toxoplasma gondii/T. gondii tachyzoites is an obligate but complex multistep process occurring in second-scale. To capture the dynamic nature of the whole entry process requires fast and high-resolution live cell imaging. Recent advances in T. gondii/host cell genome editing and in quantitative live cell imaging—image acquisition and processing included—provide a systematic way to accurately phenotype T. gondii tachyzoite invasive behaviour and to highlight any variation or default from a standard scenario. Therefore, applying these combined strategies allows gaining deeper insights into the complex mechanisms underlying host cell invasion.

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Authors and Affiliations

  1. Institute for Advanced Biosciences (IAB), Team Membrane Dynamics of Parasite-Host Cell Interactions, CNRS UMR5309, INSERM U1209, Université Grenoble Alpes, Grenoble, France

    Georgios Pavlou & Isabelle Tardieux

Authors
  1. Georgios Pavlou
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  2. Isabelle Tardieux
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Corresponding author

Correspondence to Isabelle Tardieux .

Editor information

Editors and Affiliations

  1. Division of Infectious Disease and Immune Defense, The Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, Australia

    Christopher J. Tonkin

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Pavlou, G., Tardieux, I. (2020). Phenotyping Toxoplasma Invasive Skills by Fast Live Cell Imaging. In: Tonkin, C. (eds) Toxoplasma gondii. Methods in Molecular Biology, vol 2071. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9857-9_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9857-9_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9856-2

  • Online ISBN: 978-1-4939-9857-9

  • eBook Packages: Springer Protocols

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