Abstract
Staphylococcal protein A (SPA) consists of Fc-partial-region, X-region, and C-terminal. Spa typing is a genotyping method based on the presence of gene polymorphism in a variable number of 24 bp repeat sequences in the X-region. Spa typing allows highly discriminatory and rapid characterization and prediction of multilocus sequence typing (MLST), ribotyping, and pulsed-field gel electrophoresis (PFGE). DNA sequence-based approaches are becoming more frequently used because of the ease with which sequence data can be transferred between laboratories via the Internet.
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References
Grundmann H, Hori S, Enright MC, Webster C, Tami A et al (2002) Determining the genetic structure of the natural population of Staphylococcus aureus: a comparison of multilocus sequence typing with pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing. J Clin Microbiol 40:4544–4546
Feil EJ, Cooper JE, Grundmann H, Robinson DA, Enright MC et al (2003) How clonal is Staphylococcus aureus? J Bacteriol 185:3307–3316
Struelens MJ, Hawkey PM, French GL, Witte W, Tacconelli E (2009) Laboratory tools and strategies for methicillin-resistant Staphylococcus aureus screening, surveillance and typing: state of the art and unmet needs. Clin Microbiol Infect 15:112–119
HM F, AE B, LM S, vL WJ, CM VG et al (1996) Molecular typing of methicillin-resistant Staphylococcus aureus on the basis of protein A gene polymorphism. Eur J Clin Microbiol Infect Dis 15:60–64
Strommenger B, Kettlitz C, Weniger T, Harmsen D, Friedrich AW et al (2006) Assignment of Staphylococcus isolates to groups by spa typing, SmaI macrorestriction analysis, and multilocus sequence typing. J Clin Microbiol 44:2533–2540
Koreen L, Ramaswamy SV, Graviss EA, Naidich S, Musser JA et al (2004) spa typing method for discriminating among Staphylococcus aureus isolates: implications for use of a single marker to detect genetic micro- and macrovariation. J Clin Microbiol 42:792–799
Mellmann A, Weniger T, Berssenbrugge C, Rothganger J, Sammeth M et al (2007) Based upon repeat pattern (BURP): an algorithm to characterize the long-term evolution of Staphylococcus aureus populations based on spa polymorphisms. BMC Microbiol 7:98
Acknowledgment
We thank Yingjie Wu and Kai Wang at AuGCT DNA-SYN Biotechnology Co. Ltd. (Yangling) for data analysis of the cleaned PCR product, sequencing reaction, and DNA sequencing.
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Wang, X. (2020). Spa Typing of Staphylococcus aureus Isolates. In: Ji, Y. (eds) Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols. Methods in Molecular Biology, vol 2069. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9849-4_6
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DOI: https://doi.org/10.1007/978-1-4939-9849-4_6
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