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Using Toxins in Brain Slice Recordings

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2068))

Abstract

Use of biological toxins from different kinds is widely accepted in electrophysiological experiments. In particular, electrophysiological recordings from brain tissue slices are usually conducted with toxins to manipulate on different receptors or ion channels. Here we describe usage of toxins in electrophysiological experiments in acute brain slices.

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Notes

  1. 1.

    Glass electrode preparation is not discussed in this chapter.

References

  1. Verkhratsky A, Parpura V (2014) History of electrophysiology and the patch clamp, pp 1–19

    Google Scholar 

  2. Neher E, Sakmann B (1976) Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature 260:799–802

    Article  CAS  Google Scholar 

  3. Eccles JC (1966) The ionic mechanisms of excitatory and inhibitory synaptic action. Ann N Y Acad Sci 137:473–494

    Article  CAS  Google Scholar 

  4. Brazier MAB (1964) The electrical activity of the nervous system: electrical signals are the neurophysiologist’s clue to coding in the nervous system. Science 146(80):1423–1428

    Article  CAS  Google Scholar 

  5. Hodgkin AL, Huxley AF (1939) Action potentials recorded from inside a nerve fibre. Nature 144:710–711

    Article  Google Scholar 

  6. Hamill OP, Marty A, Neher E et al (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch Eur J Physiol 391:85–100

    Article  CAS  Google Scholar 

  7. Sharott A (2014) Local field potential, methods of recording. In: Encyclopedia of computational neuroscience. Springer, New York, NY, pp 1–3

    Google Scholar 

  8. Zufall F (1992) Neurotoxins as Tools in Characterization of γ-Aminobutyric Acid-Activated Chloride Channels. In: Methods in Neurosciences Volume 8. ACADEMIC PRESS, INC., pp 323–331

    Google Scholar 

  9. Newland CF, Cull-Candy SG (1992) On the mechanism of action of picrotoxin on GABA receptor channels in dissociated sympathetic neurones of the rat. J Physiol 447:191–213

    Article  CAS  Google Scholar 

  10. Roy ML, Narahashi T (1994) Sodium channels of rat dorsal root ganglion neurons, pp 21–38

    Google Scholar 

  11. Chen R, Chung S-H (2014) Mechanism of tetrodotoxin block and resistance in sodium channels. Biochem Biophys Res Commun 446:370–374

    Article  CAS  Google Scholar 

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Correspondence to Rami Yaka .

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Bingor, A., Yaka, R. (2020). Using Toxins in Brain Slice Recordings. In: Priel, A. (eds) Snake and Spider Toxins. Methods in Molecular Biology, vol 2068. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9845-6_14

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  • DOI: https://doi.org/10.1007/978-1-4939-9845-6_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9844-9

  • Online ISBN: 978-1-4939-9845-6

  • eBook Packages: Springer Protocols

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