Skip to main content

Embryo Transfer Surgery

Part of the Methods in Molecular Biology book series (MIMB,volume 2066)


Embryo transfer surgery is an essential step in the process of generating gene-modified mice, regardless of whether the embryos were modified by DNA, RNA CRISPR components, or embryonic stem cells, or whether they were microinjected or electroporated. Transfer is also a necessary step for assisted reproductive techniques such as rederivation and reanimation by in vitro fertilization. The manipulated embryos must be returned to the reproductive tract of a pseudopregnant recipient female mouse, wherein the transplanted embryos develop to term. Embryos may be transferred to either the oviduct or uterine horn, depending upon the developmental status of the embryos and the stage of the recipient. This chapter will describe the process of transferring embryos surgically to a recipient female.

Key words

  • Embryo transfer
  • Surgery
  • Mouse
  • Recipient
  • Rederivation
  • Pseudopregnant
  • Vasectomized male

This is a preview of subscription content, access via your institution.

Buying options

USD   49.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-1-4939-9837-1_8
  • Chapter length: 6 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
USD   109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-9837-1
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   149.99
Price excludes VAT (USA)
Hardcover Book
USD   219.99
Price excludes VAT (USA)

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more


  1. Gunnet JW, Freeman ME (1984) Hypothalamic regulation of mating-induced prolactin release. Effect of electrical stimulation of the medial preoptic area in conscious female rats. Neuroendo 38:12–16

    CAS  CrossRef  Google Scholar 

  2. Osada T, Watanabe G, Sakaki Y, Takeuchi T (2001) Puromycin-sensitive aminopeptidase is essential for the maternal recognition of pregnancy in mice. Mol Endocrinol 15(6):882–893

    CAS  CrossRef  Google Scholar 

  3. McLaren A, Michie D (1956) Studies on the transfer of fertilized mouse eggs to uterine foster mothers 1. Factors affecting the implantation and survival of native and transferred eggs. J Exp Biol 33:394–416

    Google Scholar 

  4. Byers SL, Wiles MV, Dunn SL, Taft RA (2012) Mouse estrous cycle identification tool and images. PLoS One 7(4):e35538. PMID: 22514749

    CAS  CrossRef  Google Scholar 

  5. Zeller W, Meier G, Burki K, Panoussis B (1998) Adverse effects of tribromoethanol as used in the production of transgenic mice. Lab Anim 32(4):407–413

    CAS  CrossRef  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Melissa A. Larson .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 2020 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Verify currency and authenticity via CrossMark

Cite this protocol

Larson, M.A. (2020). Embryo Transfer Surgery. In: Larson, M. (eds) Transgenic Mouse. Methods in Molecular Biology, vol 2066. Humana, New York, NY.

Download citation

  • DOI:

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9836-4

  • Online ISBN: 978-1-4939-9837-1

  • eBook Packages: Springer Protocols