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Pronuclear Microinjection of One-Cell Embryos

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Part of the Methods in Molecular Biology book series (MIMB,volume 2066)

Abstract

Generating a transgenic or gene-modified mouse requires the introduction of exogenous reagents into an early-stage embryo. The mouse one-cell embryo or zygote possesses two pronuclei, representing the genetic contribution of the sperm and oocyte. Traditional transgenic mice are generated by injecting a DNA solution containing a purified transgene construct into the male pronucleus, generally the larger of the two pronuclei. Similarly, gene-editing reagents such as ZFNs, TALENs, and CRISPR RNAs are introduced into zygotes in the same manner, making this technique applicable to a wide variety of projects. This chapter presents the procedures for pronuclear microinjection.

Key words

  • Transgenic
  • CRISPR
  • Microinjection
  • Zygotes
  • In vivo
  • Gene editing

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  • DOI: 10.1007/978-1-4939-9837-1_2
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References

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Correspondence to Melissa A. Larson .

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Larson, M.A. (2020). Pronuclear Microinjection of One-Cell Embryos. In: Larson, M. (eds) Transgenic Mouse. Methods in Molecular Biology, vol 2066. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9837-1_2

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  • DOI: https://doi.org/10.1007/978-1-4939-9837-1_2

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9836-4

  • Online ISBN: 978-1-4939-9837-1

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