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Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells Using Sendai Virus

Part of the Methods in Molecular Biology book series (MIMB,volume 2066)

Abstract

Induced pluripotent stem (iPS) cells are important tools for studying differentiation and for use in patient-specific disease modeling. We present a detailed method for the reprogramming of primary human fibroblasts to induced pluripotent stem cells using Sendai virus. These procedures allow for the efficient generation of multiple high-quality feeder-independent iPS cell lines for a given human fibroblast line. The iPS cell lines generated by this protocol can be used in a variety of differentiation and gene expression studies, as well as in genetic manipulations.

Key words

  • Human pluripotent stem cells
  • Induced pluripotent stem cells
  • Reprogramming
  • Primary cell
  • Sendai virus
  • Fibroblast

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  • DOI: 10.1007/978-1-4939-9837-1_18
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Correspondence to Jay L. Vivian .

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Draper, J.M., Vivian, J.L. (2020). Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells Using Sendai Virus. In: Larson, M. (eds) Transgenic Mouse. Methods in Molecular Biology, vol 2066. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9837-1_18

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  • DOI: https://doi.org/10.1007/978-1-4939-9837-1_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9836-4

  • Online ISBN: 978-1-4939-9837-1

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