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Sperm-Mediated Genetic Modifications

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Part of the Methods in Molecular Biology book series (MIMB,volume 2066)

Abstract

The ability to introduce controlled modifications of the genome of animals represents an important tool for biomedical and veterinary research. Among transgenic techniques, we describe here the sperm-mediated gene transfer method that is based on the spontaneous ability of sperm cells to bind and internalize exogenous DNA and to carry it to the oocyte during fertilization, producing genetically modified animals.

Key words

  • Spermatozoa
  • Transgenic animals
  • SMGT
  • Transgenesis
  • Mouse

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  • DOI: 10.1007/978-1-4939-9837-1_11
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Acknowledgments

This work was supported by the Italian Minister of Research and University (FIRB, RBAP06LAHL).

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Correspondence to Marialuisa Lavitrano .

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Lavitrano, M., Farina, L., Cerrito, M.G., Giovannoni, R. (2020). Sperm-Mediated Genetic Modifications. In: Larson, M. (eds) Transgenic Mouse. Methods in Molecular Biology, vol 2066. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9837-1_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9837-1_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9836-4

  • Online ISBN: 978-1-4939-9837-1

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