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Compact Quantum Dots for Quantitative Cytology

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Single Cell Metabolism

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2064))

Abstract

In this chapter, we describe the preparation of fluorescent quantum dots for imaging and measuring protein expression in cells. Quantum dots are nanocrystals that have numerous advantages for biomolecular detection compared with organic dyes and fluorescent proteins, but their large size has been a limiting factor. We describe the synthesis of nanoparticles smaller than 10 nm (smaller than an antibody), their attachment to monoclonal antibodies through click chemistry, characterization of the conjugates, and use for labeling of cellular antigens. We further discuss the unique advantages and challenges associated with this approach compared with conventional immunofluorescence techniques.

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Authors and Affiliations

  1. Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Phuong Le, Shweta Chitoor, Chunlai Tu, Sung Jun Lim & Andrew M. Smith

  2. Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Phuong Le, Shweta Chitoor, Chunlai Tu, Sung Jun Lim & Andrew M. Smith

  3. Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Andrew M. Smith

Authors
  1. Phuong Le
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  2. Shweta Chitoor
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  3. Chunlai Tu
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  4. Sung Jun Lim
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  5. Andrew M. Smith
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Corresponding author

Correspondence to Andrew M. Smith .

Editor information

Editors and Affiliations

  1. Waters Corporation, Milford, MA, USA

    Bindesh Shrestha

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Le, P., Chitoor, S., Tu, C., Lim, S.J., Smith, A.M. (2020). Compact Quantum Dots for Quantitative Cytology. In: Shrestha, B. (eds) Single Cell Metabolism. Methods in Molecular Biology, vol 2064. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9831-9_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9831-9_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9829-6

  • Online ISBN: 978-1-4939-9831-9

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