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Sample Preparation and Analysis of Single Cells Using High Performance MALDI FTICR Mass Spectrometry

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Single Cell Metabolism

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2064))

Abstract

Imaging mass spectrometry is a powerful technology that combines the molecular measurements of mass spectrometry with the spatial information inherent to microscopy. This unique combination of capabilities is ideally suited for the analysis of metabolites and lipids from single cells. This chapter describes a methodology for the sample preparation and analysis of single cells using high performance MALDI FTICR MS. Using this approach, we are able to generate profiles of lipid and metabolite expression from single cells that characterize cellular heterogeneity. This approach also enables the detection of variations in the expression profiles of lipids and metabolites induced by chemical stimulation of the cells. These results demonstrate that MALDI IMS provides an insightful view of lipid and metabolite expression useful in the characterization of a number of biological systems at the single cell level.

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Acknowledgments

This work is supported by NIH grants R33DK105149 and P41GM103391. The authors also acknowledge Nichole A. Lobdell from Eric P. Skaar Lab for providing the cultured cells for this work.

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Authors and Affiliations

  1. Mass Spectrometry Research Center, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA

    Bo Yang, Tina Tsui, Richard M. Caprioli & Jeremy L. Norris

Authors
  1. Bo Yang
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  2. Tina Tsui
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  3. Richard M. Caprioli
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  4. Jeremy L. Norris
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Corresponding author

Correspondence to Jeremy L. Norris .

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Editors and Affiliations

  1. Waters Corporation, Milford, MA, USA

    Bindesh Shrestha

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Yang, B., Tsui, T., Caprioli, R.M., Norris, J.L. (2020). Sample Preparation and Analysis of Single Cells Using High Performance MALDI FTICR Mass Spectrometry. In: Shrestha, B. (eds) Single Cell Metabolism. Methods in Molecular Biology, vol 2064. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9831-9_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9831-9_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9829-6

  • Online ISBN: 978-1-4939-9831-9

  • eBook Packages: Springer Protocols

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