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Single-Cell Metabolomics by Mass Spectrometry

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Book cover Single Cell Metabolism

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2064))

Abstract

Single-cell level metabolomics gives a snapshot of small molecules, intermediates, and products of cellular metabolism within a biological system. These small molecules, typically less than 1 kDa in molecular weight, often provide the basis of biochemical heterogeneity within cells. The molecular differences between cells with a cell type are often attributed to random stochastic biochemical processes, cell cycle stages, environmental stress, and diseased states. In this chapter, current limitations and challenges in single-cell analysis by mass spectrometry will be discussed alongside the prospects of single-cell metabolomics in systems biology. A few selected example of the recent development in mass spectrometry tools to unravel single-cell metabolomics will be described as well.

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Authors and Affiliations

  1. Waters Corporation, Milford, MA, USA

    Bindesh Shrestha

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  1. Bindesh Shrestha
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Correspondence to Bindesh Shrestha .

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  1. Waters Corporation, Milford, MA, USA

    Bindesh Shrestha

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Shrestha, B. (2020). Single-Cell Metabolomics by Mass Spectrometry. In: Shrestha, B. (eds) Single Cell Metabolism. Methods in Molecular Biology, vol 2064. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9831-9_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9831-9_1

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9829-6

  • Online ISBN: 978-1-4939-9831-9

  • eBook Packages: Springer Protocols

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