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Analyzing Somatic DNA Repair in Arabidopsis Meiotic Mutants

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Plant Meiosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2061))

Abstract

Meiotic and somatic recombination share a common set of factors. Thus, the analysis of somatic DNA repair in meiotic mutant lines should be of special interest. Growth defects of mutant plants induced by specific genotoxins can thereby hint to DNA repair functions of the affected proteins. Here, we describe two kinds of approaches to characterize deficiencies in DNA repair in mutant lines of Arabidopsis thaliana, after genotoxin treatment.

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Acknowledgments

This work was supported by the European Research Council (Advanced Grant “COMREC” and “CRISBREED”).

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Authors and Affiliations

  1. Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe, Germany

    Annika Dorn & Holger Puchta

Authors
  1. Annika Dorn
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  2. Holger Puchta
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Corresponding author

Correspondence to Holger Puchta .

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Editors and Affiliations

  1. Departamento de Genética, Fisiología y Microbiología, Facultad de Biología, Universidad Complutense de Madrid, Madrid, Spain

    Mónica Pradillo

  2. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben, Stadt Seeland, Germany

    Stefan Heckmann

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Dorn, A., Puchta, H. (2020). Analyzing Somatic DNA Repair in Arabidopsis Meiotic Mutants. In: Pradillo, M., Heckmann, S. (eds) Plant Meiosis. Methods in Molecular Biology, vol 2061. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9818-0_25

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  • DOI: https://doi.org/10.1007/978-1-4939-9818-0_25

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9817-3

  • Online ISBN: 978-1-4939-9818-0

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