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Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication

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Herpes Simplex Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2060))

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Abstract

The possibility to label specific viral and cellular structures with live cell markers such as autofluorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows for monitoring virus replication in a spatial and temporal fashion. Here, we describe the multifluorescent live analysis of the multicompartment Herpes Simplex Virus Type-1 (HSV-1) by live cell confocal laser scanning microscopy.

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References

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Correspondence to Michael Seyffert .

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Seyffert, M., Fraefel, C. (2020). Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication. In: Diefenbach, R., Fraefel, C. (eds) Herpes Simplex Virus . Methods in Molecular Biology, vol 2060. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9814-2_22

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  • DOI: https://doi.org/10.1007/978-1-4939-9814-2_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9813-5

  • Online ISBN: 978-1-4939-9814-2

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