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Phenotypic and Genotypic Testing of HSV-1 and HSV-2 Resistance to Antivirals

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Herpes Simplex Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2060))

Abstract

Resistance testing of antivirals to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) can be done by phenotypic and genotypic methods. The determination of a resistant phenotype is based on the calculation of inhibitory concentrations for the antiviral drug, which should be tested. The main advantage of this resistance test is a clear interpretation of laboratory findings, but the method is time-consuming and a considerable experience is required by handling infectious virus. Genotypic resistance testing is based on the detection of resistance-related mutations in viral genes encoding the thymidine kinase and DNA polymerase, which need to be amplified and sequenced. This approach has the advantage of being faster, but only frameshift mutations, stops of translation, and amino acid substitutions described in the literature can be interpreted without doubt. By contrast, numerous novel amino acid substitutions are diagnostically less conclusive.

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Author information

Authors and Affiliations

  1. Section of Experimental Virology, Institute for Medical Microbiology, Jena University Hospital, Jena, Germany

    Andreas Sauerbrei

  2. Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Kathrin Bohn-Wippert

Authors
  1. Andreas Sauerbrei
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  2. Kathrin Bohn-Wippert
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Corresponding author

Correspondence to Andreas Sauerbrei .

Editor information

Editors and Affiliations

  1. Department of Biomedical Sciences, Macquarie University, Sydney, NSW, Australia

    Russell J. Diefenbach

  2. Institute of Virology, University of Zurich, Zürich, Switzerland

    Cornel Fraefel

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Sauerbrei, A., Bohn-Wippert, K. (2020). Phenotypic and Genotypic Testing of HSV-1 and HSV-2 Resistance to Antivirals. In: Diefenbach, R., Fraefel, C. (eds) Herpes Simplex Virus . Methods in Molecular Biology, vol 2060. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9814-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9814-2_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9813-5

  • Online ISBN: 978-1-4939-9814-2

  • eBook Packages: Springer Protocols

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