Abstract
Cholera toxin subunit B (CTB) is the nontoxic moiety of cholera toxin. It can target the glycosphingolipid GM1 expressed in the blood–brain barrier (BBB), neovasculature, and glioblastoma cells. Thus, CTB has been utilized as a multifunctional molecule for targeted therapy of glioblastoma. Here, we describe a detailed method for preparation of CTB functionalized paclitaxel (PTX)-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles. This unique modification can guide nanoparticles across the BBB and target glioblastoma cells. The characterization of nanoparticles such as size, zeta potential, morphology, drug loading, and encapsulation efficiency is shown in this chapter.
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Acknowledgment
This work was supported by the National Natural Science Foundation of China (81673361, 81690263, and 81673370) and Shanghai Natural Science Foundation (18ZR1404800).
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Guan, J., Qian, J., Zhan, C. (2020). Preparation of Cholera Toxin Subunit B Functionalized Nanoparticles for Targeted Therapy of Glioblastoma. In: Jain, K. (eds) Drug Delivery Systems. Methods in Molecular Biology, vol 2059. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9798-5_10
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DOI: https://doi.org/10.1007/978-1-4939-9798-5_10
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9797-8
Online ISBN: 978-1-4939-9798-5
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