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Isolation and Quantification of MicroRNAs from Human Saliva

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Theranostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2054))

Abstract

Oral premalignant disorders (OPMD) have relatively high malignant transformation rates to Oral Cancers (OC). Oral carcinogenesis is a multistep process that originates as epithelial hyperplasia followed by epithelial dysplasia, leading to fully malignant phenotypes. Early detection can be lifesaving but is currently not possible due to the lack of early diagnostic tools. The current diagnostic methods such as biopsy sampling, tumor tissue staining, and imaging techniques require skilled personnel and are invasive, painful, time-consuming, and expensive. Saliva has gained momentum as the diagnostic fluid of the future due to its noninvasive nature, ease of sampling, multiple samples can be collected with ease and more importantly does not require skilled personnel. The use of saliva in cancer diagnostics is an emerging and an expanding field. MicroRNA (miRNA) play a role in cancer initiation and progression and the expression changes of miRNA have been investigated as a potential biomarker in cancer studies. In this chapter, we describe a robust and cost-effective protocol to isolate and enrich miRNA from saliva samples. Profiling miRNAs in saliva samples can form part of the clinical management of OPMD and OC patients in the future.

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Acknowledgments

Our work was supported by the Janssen: Pharmaceutical Companies of Johnson & Johnson and Translational Research Institute (TRI) Spore Grant.

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Authors and Affiliations

  1. School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD, Australia

    Sri Ram Arunachalam

  2. The School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD, Australia

    Sri Ram Arunachalam, Kai Dun Tang & Chamindie Punyadeera

  3. Translational Research Institute, Brisbane, QLD, Australia

    Chamindie Punyadeera

Authors
  1. Sri Ram Arunachalam
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  2. Kai Dun Tang
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  3. Chamindie Punyadeera
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Corresponding author

Correspondence to Chamindie Punyadeera .

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Editors and Affiliations

  1. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Jyotsna Batra

  2. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Srilakshmi Srinivasan

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Arunachalam, S.R., Tang, K.D., Punyadeera, C. (2019). Isolation and Quantification of MicroRNAs from Human Saliva. In: Batra, J., Srinivasan, S. (eds) Theranostics. Methods in Molecular Biology, vol 2054. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9769-5_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9769-5_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9768-8

  • Online ISBN: 978-1-4939-9769-5

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