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Nucleic Acid Aptamers as Emerging Tools for Diagnostics and Theranostics

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Book cover Theranostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2054))

Abstract

Aptamers are ssDNA or RNA sequences (20–80 nucleotides) generated in vitro by SELEX (Systematic Evolution of Ligands using EXponential enrichment) against diverse range of targets from small molecules to bacteria, viruses, and even eukaryotic cells. Aptamers, also known as chemical bodies, bind to their respective targets with tunable affinity and specificity, making aptamers as potent probes for diagnostics and excellent ligands for drug delivery in therapeutics. In this chapter, we have described the methods for generating DNA aptamers against proteins and their use in theranostics.

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Acknowledgments

The aptamer work in Navani group is supported by Department of Biotechnology grant, India and DST, India. The authors acknowledge Shubham Jain for his assistance in drawing schematics. R.M. is supported by SERB-NPDF.

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Authors and Affiliations

  1. Chemical Biology Lab, Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee, India

    Ruchi Mutreja, Shahnawaz Ahmad Baba & Naveen Kumar Navani

Authors
  1. Ruchi Mutreja
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  2. Shahnawaz Ahmad Baba
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  3. Naveen Kumar Navani
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Corresponding author

Correspondence to Naveen Kumar Navani .

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Editors and Affiliations

  1. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Jyotsna Batra

  2. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Srilakshmi Srinivasan

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Mutreja, R., Baba, S.A., Navani, N.K. (2019). Nucleic Acid Aptamers as Emerging Tools for Diagnostics and Theranostics. In: Batra, J., Srinivasan, S. (eds) Theranostics. Methods in Molecular Biology, vol 2054. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9769-5_14

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  • DOI: https://doi.org/10.1007/978-1-4939-9769-5_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9768-8

  • Online ISBN: 978-1-4939-9769-5

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