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Subcellular Localization of MicroRNAs by MicroRNA In Situ Hybridization (miR-ISH)

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Theranostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2054))

Abstract

MicroRNAs (miRNAs) are 22-nucleotide RNA sequences that regulate up to 60% of the mammalian transcriptome. Although canonical miRNA-induced silencing complex-mediated messenger RNA degradation occurs in the cytoplasm, miRNAs have been described in other subcellular compartments with potentially novel functions. Currently, there are limited methodologies for visualizing RNA locations within cells to elucidate mechanisms and pathways of miRNA biogenesis, transport, and function. Here, we describe a simple and rapid miRNA in situ hybridization method that can be combined with standard immunofluorescence procedures for subcellular localization of mature and precursor miRNAs.

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Author information

Authors and Affiliations

  1. QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia

    Harley Rose Robinson & Michelle Mei Chih Hill

  2. UQ Diamantina Institute, The University of Queensland, Woolloongabba, QLD, Australia

    Harley Rose Robinson, Michelle Mei Chih Hill & Alexandre Santos Cristino

  3. Griffith Institute for Drug Discovery, Griffith University, Brisbane, QLD, Australia

    Alexandre Santos Cristino

Authors
  1. Harley Rose Robinson
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  2. Michelle Mei Chih Hill
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  3. Alexandre Santos Cristino
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Corresponding author

Correspondence to Alexandre Santos Cristino .

Editor information

Editors and Affiliations

  1. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Jyotsna Batra

  2. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Srilakshmi Srinivasan

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Robinson, H.R., Hill, M.M.C., Cristino, A.S. (2019). Subcellular Localization of MicroRNAs by MicroRNA In Situ Hybridization (miR-ISH). In: Batra, J., Srinivasan, S. (eds) Theranostics. Methods in Molecular Biology, vol 2054. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9769-5_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9769-5_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9768-8

  • Online ISBN: 978-1-4939-9769-5

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