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Reactive Oxygen Species Detection of Drosophila Cells by Flow Cytometry

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Fundamental Approaches to Screen Abnormalities in Drosophila

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Flow cytometry is an accurate and versatile technique to measure the fluorescence intensity of a large population of cells. In this chapter, we are using flow cytometer to detect the number of cells producing reactive oxygen species (ROS) in Drosophila under stress condition. ROS constitute a group of reactive molecules and free radicals derived from molecular oxygen. In this protocol, we are describing estimation of ROS from various types of Drosophila larval tissues, like imaginal disc, gut and freshly isolated hemolymph.

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Acknowledgements

We are thankful to Swetapadma Sahu and Gyanaseni Dhar for standardization of the experiment. RR is thankful to DST WOS-A for financial support. SB is thankful to Odisha DBT 3325/ST(BIO)-02/2017. MM lab is supported by Grant No. BT/PR21857/NNT/28/1238/2017, EMR/2017/003054 and Odisha DBT 3325/ST(BIO)-02/2017.

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Authors and Affiliations

  1. Neural Developmental Biology Lab, Department of Life Science, National Institute of Technology Rourkela, Rourkela, Odisha, India

    Ronnaly Rout, Srirupa Basu & Monalisa Mishra

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  1. Ronnaly Rout
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  2. Srirupa Basu
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  3. Monalisa Mishra
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Correspondence to Monalisa Mishra .

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Editors and Affiliations

  1. Neural Developmental Biology Lab, Department of Life Science, National Institute of Technology Rourkela, Rourkela, Odisha, India

    Monalisa Mishra

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Rout, R., Basu, S., Mishra, M. (2020). Reactive Oxygen Species Detection of Drosophila Cells by Flow Cytometry. In: Mishra, M. (eds) Fundamental Approaches to Screen Abnormalities in Drosophila. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-9756-5_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9756-5_9

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-9755-8

  • Online ISBN: 978-1-4939-9756-5

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