Abstract
Drosophila hemolymph is analogous to vertebrate blood. It also provides an innate immune system which possesses three different types of cells. The cells stain with Giemsa solution which is composed of Azure B, eosin and methylene blue. This stain is usually used to stain the blood samples to identify the various blood cell types. Mostly it stains the G-banding of chromosomes, where it strongly binds with the phosphate group of DNA. In human blood cells, erythrocytes stain pink, monocyte cytoplasm stains pale blue, platelets stain light pale pink, leukocytes and nuclear chromatin stain magenta and lymphocyte cytoplasm stains sky blue. In Drosophila hemolymph, three types of cells are found. These are plasmatocytes, crystallocytes and lamellocytes. Where plasmatocytes stain with light pink stain, crystal cells stain dark blue, and lamellocytes stain light cytoplasm with dark nuclei. The current study explains the analysis of haemocyte of Drosophila.
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References
Shank R, Aprison M (1981) Present status and significance of the glutamine cycle in neural tissues. Life Sci 28(8):837–842
Piyankarage SC, Augustin H, Grosjean Y, Featherstone DE, Shippy SA (2008) Hemolymph amino acid analysis of individual Drosophila larvae. Anal Chem 80(4):1201–1207
Sorrentino RP, Melk JP, Govind S (2004) Genetic analysis of contributions of dorsal group and JAK-Stat92E pathway genes to larval hemocyte concentration and the egg encapsulation response in Drosophila. Genetics 166(3):1343–1356
Ratcliffe N (1979) Role of hemocytes in defense against biological agents. Insect hemocytes: development, forms, functions, and techniques, pp 331–414
Hultmark D (2003) Drosophila immunity: paths and patterns. Curr Opin Immunol 15(1):12–19
Hoffmann JA (2003) The immune response of Drosophila. Nature 426(6962):33
Wyatt GR (1961) The biochemistry of insect hemolymph. Annu Rev Entomol 6(1):75–102
Choma MA, Suter MJ, Vakoc BJ, Bouma BE, Tearney GJ (2011) Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems. Dis Model Mech 4(3):411–420
Franc NC, Dimarcq J-L, Lagueux M, Hoffmann J, Ezekowitz RAB (1996) Croquemort, a novel Drosophila hemocyte/macrophage receptor that recognizes apoptotic cells. Immunity 4(5):431–443
Hetru C, Troxler L, Hoffmann JA (2003) Drosophila melanogaster antimicrobial defense. J Infect Dis 187(Supplement_2):S327–S334
Sackton TB, Lazzaro BP, Schlenke TA, Evans JD, Hultmark D, Clark AG (2007) Dynamic evolution of the innate immune system in Drosophila. Nat Genet 39(12):1461
Makhijani K, Brückner K (2012) Of blood cells and the nervous system: hematopoiesis in the Drosophila larva. Fly 6(4):254–260
Jung S-H, Evans CJ, Uemura C, Banerjee U (2005) The Drosophila lymph gland as a developmental model of hematopoiesis. Development 132(11):2521–2533
Pearson AM, Baksa K, Rämet M, Protas M, McKee M, Brown D, Ezekowitz RAB (2003) Identification of cytoskeletal regulatory proteins required for efficient phagocytosis in Drosophila. Microbes Infect 5(10):815–824
Holz A, Bossinger B, Strasser T, Janning W, Klapper R (2003) The two origins of hemocytes in Drosophila. Development 130(20):4955–4962
Lanot R, Zachary D, Holder F, Meister M (2001) Postembryonic hematopoiesis in Drosophila. Dev Biol 230(2):243–257
Márkus R, Laurinyecz B, Kurucz É, Honti V, Bajusz I, Sipos B, Somogyi K, Kronhamn J, Hultmark D, Andó I (2009) Sessile hemocytes as a hematopoietic compartment in Drosophila melanogaster. Proc Natl Acad Sci 106(12):4805–4809
Perry P, Wolff S (1974) New Giemsa method for the differential staining of sister chromatids. Nature 251(5471):156
Fischer AH, Jacobson KA, Rose J, Zeller R (2008) Hematoxylin and eosin staining of tissue and cell sections. Cold Spring Harb Protoc 2008(5):pdb. prot4986
van Furth R, Raeburn JA, van Zwet TL (1979) Characteristics of human mononuclear phagocytes. Blood 54(2):485–500
Silva J, Boleli I, Simões Z (2002) Hemocyte types and total and differential counts in unparasitized and parasitized Anastrepha obliqua (Diptera, Tephritidae) larvae. Braz J Biol 62(4A):689–699
Meister M, Lagueux M (2003) Drosophila blood cells. Cell Microbiol 5(9):573–580
Meister M (2004) Blood cells of Drosophila: cell lineages and role in host defence. Curr Opin Immunol 16(1):10–15
Acknowledgements
JB is thankful to BT/PR21857/NNT/28/1238/2017 for financial support. MM lab is supported by Grant No. BT/PR21857/NNT/28/1238/2017, EMR/2017/003054, Odisha DBT 3325/ST(BIO)-02/2017.
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Bag, J., Mishra, M. (2020). Hemolymph Analysis of Drosophila melanogaster by Giemsa Staining. In: Mishra, M. (eds) Fundamental Approaches to Screen Abnormalities in Drosophila. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-9756-5_3
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DOI: https://doi.org/10.1007/978-1-4939-9756-5_3
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