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Isolation of Gut, Imaginal Disc, Fat Body, Ovary and Testes in Various Developmental Stages of Drosophila

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Fundamental Approaches to Screen Abnormalities in Drosophila

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Abstract

Drosophila melanogaster shares a remarkable resemblance in terms of its organ anatomy and function with that of mammals. Various organs of the fly are used as a model to study its pathologies and metabolic and therapeutic purposes. Gut, the largest organ of the fly body, shares similarity with mammalian intestine in terms of metabolic pathways, physiology and signalling. The fat body of the fly resembles the liver of mammals and helps in fat storage and nutrient sensing. Imaginal disc composed of 20–40 cells are used to check defect in early time point of development. The testes and ovary are used to study the reproductive defect. This chapter describes the dissection of the gut, fat body, ovary, testes, and imaginal discs from larvae and adult tissue.

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Acknowledgements

NN and GD are thankful to DST/INSPIRE Fellowship for financial support. MM lab is supported by Grant No. BT/PR21857/NNT/28/1238/2017, EMR/2017/003054, Odisha DST 3325/ST(BIO)-02/2017.

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  1. Neural Developmental Biology Lab, Department of Life Science, National Institute of Technology Rourkela, Rourkela, Odisha, India

    Nibedita Nayak, Gyanaseni Dhar & Monalisa Mishra

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  1. Nibedita Nayak
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  2. Gyanaseni Dhar
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  3. Monalisa Mishra
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Correspondence to Monalisa Mishra .

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  1. Neural Developmental Biology Lab, Department of Life Science, National Institute of Technology Rourkela, Rourkela, Odisha, India

    Monalisa Mishra

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Nayak, N., Dhar, G., Mishra, M. (2020). Isolation of Gut, Imaginal Disc, Fat Body, Ovary and Testes in Various Developmental Stages of Drosophila. In: Mishra, M. (eds) Fundamental Approaches to Screen Abnormalities in Drosophila. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-9756-5_2

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