Abstract
Fourier transform infrared (FTIR) spectroscopy is the technique which allows the analysis of a biological sample or biofluid to characterize the functional group. It reveals the molecular information without disturbing the chemical nature of the biological sample. It doesn’t require any labeling. We have used vibrational spectroscopic techniques to analyse the functional group of hemolymph. We used various treatments in flies to see the alteration of functional group in the hemolymph. Flies were fed with bacteria-contaminated food and nanoparticle (alumina NP and lignin NP) mixed food to check the alteration of the biochemical composition of hemolymph. Parameters like the composition of lipid, protein, nucleic acid and DNA undergo changes with respect to chemicals. The current protocol describes the analysis of carbohydrate, lipid, protein, nucleic acid and DNA damage from the hemolymph using FTIR.
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Acknowledgements
SP is thankful to MHRD for the financial support. MM lab is supported by Grant No. BT/PR21857/NNT/28/1238/2017, EMR/2017/003054, Odisha DBT 3325/ST (BIO)-02/2017.
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Paikra, S., Mishra, M. (2020). Fourier Transform Infrared Spectroscopy: A Novel Approach for Biomolecular Characterization of Drosophila Hemolymph. In: Mishra, M. (eds) Fundamental Approaches to Screen Abnormalities in Drosophila. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-9756-5_17
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DOI: https://doi.org/10.1007/978-1-4939-9756-5_17
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