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Molecular Approaches for Analysis of Drosophila MicroRNAs

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Fundamental Approaches to Screen Abnormalities in Drosophila

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Abstract

MicroRNAs (miRNAs) belong to a class of small non-coding endogenous RNAs that regulate gene expression at the post-transcriptional level. These small RNAs recognize sequences within 3′ untranslated regions of target mRNAs in complexes referred to as a miRNA-induced silencing complex (miRISC). Drosophila melanogaster has served as an indispensable model system for defining the diverse biological roles of miRNAs, their mechanism of action as well as the role of miRNA biogenesis factors. In this chapter we describe some of the assays used for molecular analysis of Drosophila miRNAs.

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Acknowledgements

We would like to acknowledge the efforts of all researchers involved in establishing and refining the techniques and assays used to analyse miRNA function. This work was supported by the Wellcome Trust/DBT India Alliance Fellowship [grant number IA/I(S)/17/1/503085] awarded to GC.

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Authors and Affiliations

  1. RNA Biology Laboratory, Regional Centre for Biotechnology, Faridabad, India

    Manish Pandey, Sakshi Bansal & Geetanjali Chawla

Authors
  1. Manish Pandey
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  2. Sakshi Bansal
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  3. Geetanjali Chawla
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Corresponding author

Correspondence to Geetanjali Chawla .

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Editors and Affiliations

  1. Neural Developmental Biology Lab, Department of Life Science, National Institute of Technology Rourkela, Rourkela, Odisha, India

    Monalisa Mishra

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Pandey, M., Bansal, S., Chawla, G. (2020). Molecular Approaches for Analysis of Drosophila MicroRNAs. In: Mishra, M. (eds) Fundamental Approaches to Screen Abnormalities in Drosophila. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-9756-5_14

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  • DOI: https://doi.org/10.1007/978-1-4939-9756-5_14

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