Abstract
Cryptosporidium genotyping has made significant contributions to the species structure and population genetics of Cryptosporidium spp. In addition, the standardized method has shown utility in epidemiologic investigations identifying case linkages and contamination sources that could not be determined with traditional epidemiologic tools. The standardized method for Cryptosporidium genotyping from stool specimens described here comprises multiple individual protocols to amplify and sequence regions of the SSU rRNA and gp60 genes of Cryptosporidium.
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References
Ryan U, Fayer R, Xiao L (2014) Cryptosporidium species in humans and animals: current understanding and research needs. Parasitology 141(13):1667–1685. https://doi.org/10.1017/S0031182014001085
Morgan-Ryan UM, Fall A, Ward LA, Hijjawi N, Sulaiman I, Fayer R, Thompson RC, Olson M, Lal A, Xiao L (2002) Cryptosporidium hominis n. sp. (Apicomplexa: Cryptosporidiidae) from Homo sapiens. J Eukaryot Microbiol 49(6):433–440
Chalmers RM, Smith R, Elwin K, Clifton-Hadley FA, Giles M (2011) Epidemiology of anthroponotic and zoonotic human cryptosporidiosis in England and Wales, 2004-2006. Epidemiol Infect 139(5):700–712. https://doi.org/10.1017/S0950268810001688
Blackburn BG, Mazurek JM, Hlavsa M, Park J, Tillapaw M, Parrish M, Salehi E, Franks W, Koch E, Smith F, Xiao L, Arrowood M, Hill V, da Silva A, Johnston S, Jones JL (2006) Cryptosporidiosis associated with ozonated apple cider. Emerg Infect Dis 12(4):684–686. https://doi.org/10.3201/eid1204.050796
Fill MA, Lloyd J, Chakraverty T, Sweat D, Manners J, Garman K, Hlavsa MC, Roellig DM, Dunn JR, Schaffner W, Jones TF (2017) Cryptosporidiosis outbreak associated with a single hotel. J Environ Health 79(9):16–22
Hlavsa MC, Roellig DM, Seabolt MH, Kahler AM, Murphy JL, McKitt TK, Geeter EF, Dawsey R, Davidson SL, Kim TN, Tucker TH, Iverson SA, Garrett B, Fowle N, Collins J, Epperson G, Zusy S, Weiss JR, Komatsu K, Rodriguez E, Patterson JG, Sunenshine R, Taylor B, Cibulskas K, Denny L, Omura K, Tsorin B, Fullerton KE, Xiao L (2017) Using molecular characterization to support investigations of aquatic facility-associated outbreaks of cryptosporidiosis - Alabama, Arizona, and Ohio, 2016. MMWR Morb Mortal Wkly Rep 66(19):493–497. https://doi.org/10.15585/mmwr.mm6619a2
Feng Y, Tiao N, Li N, Hlavsa M, Xiao L (2014) Multilocus sequence typing of an emerging Cryptosporidium hominis subtype in the United States. J Clin Microbiol 52(2):524–530. https://doi.org/10.1128/JCM.02973-13
Xiao L, Hlavsa MC, Yoder J, Ewers C, Dearen T, Yang W, Nett R, Harris S, Brend SM, Harris M, Onischuk L, Valderrama AL, Cosgrove S, Xavier K, Hall N, Romero S, Young S, Johnston SP, Arrowood M, Roy S, Beach MJ (2009) Subtype analysis of Cryptosporidium specimens from sporadic cases in Colorado, Idaho, New Mexico, and Iowa in 2007: widespread occurrence of one Cryptosporidium hominis subtype and case history of an infection with the Cryptosporidium horse genotype. J Clin Microbiol 47(9):3017–3020. https://doi.org/10.1128/JCM.00226-09
Xiao L, Alderisio K, Limor J, Royer M, Lal AA (2000) Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool. Appl Environ Microbiol 66(12):5492–5498
Xiao L, Fayer R, Ryan U, Upton SJ (2004) Cryptosporidium taxonomy: recent advances and implications for public health. Clin Microbiol Rev 17(1):72–97
Alves M, Xiao L, Sulaiman I, Lal AA, Matos O, Antunes F (2003) Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal. J Clin Microbiol 41(6):2744–2747
Sulaiman IM, Hira PR, Zhou L, Al-Ali FM, Al-Shelahi FA, Shweiki HM, Iqbal J, Khalid N, Xiao L (2005) Unique endemicity of cryptosporidiosis in children in Kuwait. J Clin Microbiol 43(6):2805–2809. https://doi.org/10.1128/JCM.43.6.2805-2809.2005
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Disclosures: Use of trade names is for identification only and does not imply endorsement by the Public Health Service or by the U.S. Department of Health and Human Services. The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention.
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Roellig, D.M., Xiao, L. (2020). Cryptosporidium Genotyping for Epidemiology Tracking. In: Mead, J., Arrowood, M. (eds) Cryptosporidium. Methods in Molecular Biology, vol 2052. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9748-0_7
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DOI: https://doi.org/10.1007/978-1-4939-9748-0_7
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