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Low-Voltage Flow-Through Electroporation Membrane and Method

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Electroporation Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2050))

Abstract

Electroporation uses high electric field gradients to create pores within the membrane of living cells in order to deliver a substance, for example a gene, into the cytoplasm. To achieve such gradients, current electroporation devices deliver voltage pulses in the kV range to the cell medium. We describe here a new device based on gold-microtube membranes that can accomplish electroporation with voltage pulses that are orders of magnitude smaller, 4 V. The percentages of electroporated bacteria were found to be more than an order of magnitude higher than obtained with a commercial electroporator.

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Acknowledgments

This work was supported by the University of Florida.

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Authors and Affiliations

  1. Department of Chemistry, University of Florida, Gainesville, FL, USA

    Juliette Experton, Aaron G. Wilson & Charles R. Martin

Authors
  1. Juliette Experton
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  2. Aaron G. Wilson
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  3. Charles R. Martin
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Editor information

Editors and Affiliations

  1. MD Anderson Cancer Center, The University of Texas, Houston, TX, USA

    Shulin Li

  2. School of Biological Science and Medical Engineering, Beihang University, Beijing, China, Institute of Nanotechnology for Single Cell Analysis (INSCA), Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University,, Beijing, China

    Lingqian Chang

  3. Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France

    Justin Teissie

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Experton, J., Wilson, A.G., Martin, C.R. (2020). Low-Voltage Flow-Through Electroporation Membrane and Method. In: Li, S., Chang, L., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 2050. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9740-4_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9740-4_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9739-8

  • Online ISBN: 978-1-4939-9740-4

  • eBook Packages: Springer Protocols

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