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Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal

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Electroporation Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2050))

Abstract

The green alga Chlamydomonas reinhardtii has been widely used to study many biological processes, including photosynthesis, flagellar motility, sexual reproduction, metabolism, and genetics. Here, we describe a step-by-step protocol of rapid and efficient transformation method for wild type cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to the transformation of other industrially useful algae including diatom by optimizing the electric conditions.

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Acknowledgments

This work was partly supported by Advanced Low Carbon Technology Research and Development Program (ALCA, JPMJAL1105) and by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (25120714 and 16K07399).

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Authors and Affiliations

  1. Graduate School of Biostudies, Kyoto University, Kyoto, Japan

    Takashi Yamano & Hideya Fukuzawa

Authors
  1. Takashi Yamano
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  2. Hideya Fukuzawa
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Corresponding author

Correspondence to Hideya Fukuzawa .

Editor information

Editors and Affiliations

  1. MD Anderson Cancer Center, The University of Texas, Houston, TX, USA

    Shulin Li

  2. School of Biological Science and Medical Engineering, Beihang University, Beijing, China, Institute of Nanotechnology for Single Cell Analysis (INSCA), Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University,, Beijing, China

    Lingqian Chang

  3. Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France

    Justin Teissie

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Yamano, T., Fukuzawa, H. (2020). Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal. In: Li, S., Chang, L., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 2050. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9740-4_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9740-4_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9739-8

  • Online ISBN: 978-1-4939-9740-4

  • eBook Packages: Springer Protocols

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