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Electroporation Protocols pp 113–120Cite as

Targeted In Vivo Electroporation Using Nanoengineered Microelectrodes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2050))

Abstract

Targeted electroporation by using glass microelectrodes is a popular and versatile tool allowing for easy manipulation of single cells and cell ensembles in living tissue. Because of the highly focal distribution of the electric field, however, the range of reversible electroporation without causing irreversible damage is tight—especially when aiming for larger electroporation volumes. In this chapter, we describe the production of nanoengineered electroporation microelectrodes (NEMs), a practicable way to prepare glass microelectrodes providing a more even distribution around the tip of a pipette by using nanotechnological methods.

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Author information

Authors and Affiliations

  1. Behavioural Neurophysiology, Max-Planck-Institute for Medical Research, Heidelberg, Germany

    Daniel Schwarz & Andreas T. Schaefer

  2. Department of Neuroradiology, Heidelberg University Hospital, Heidelberg, Germany

    Daniel Schwarz

  3. Department of Anatomy and Cell Biology, Faculty of Medicine, University of Heidelberg, Heidelberg, Germany

    Daniel Schwarz & Andreas T. Schaefer

  4. Neurophysiology of Behaviour Laboratory, The Francis Crick Institute, London, UK

    Andreas T. Schaefer

  5. Department of Neuroscience, Physiology and Pharmacology, University College London, London, UK

    Andreas T. Schaefer

Authors
  1. Daniel Schwarz
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  2. Andreas T. Schaefer
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Corresponding author

Correspondence to Andreas T. Schaefer .

Editor information

Editors and Affiliations

  1. MD Anderson Cancer Center, The University of Texas, Houston, TX, USA

    Shulin Li

  2. School of Biological Science and Medical Engineering, Beihang University, Beijing, China, Institute of Nanotechnology for Single Cell Analysis (INSCA), Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University,, Beijing, China

    Lingqian Chang

  3. Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France

    Justin Teissie

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Schwarz, D., Schaefer, A.T. (2020). Targeted In Vivo Electroporation Using Nanoengineered Microelectrodes. In: Li, S., Chang, L., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 2050. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9740-4_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9740-4_12

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9739-8

  • Online ISBN: 978-1-4939-9740-4

  • eBook Packages: Springer Protocols

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