Abstract
Electroporation is a common method of transfection due to its relatively low risk and high transfection efficiency. The most common method of electroporation is bulk electroporation which is easily performed on large quantities of cells yet results in variable levels of viability and transfection efficiency across the population. Localized electroporation is an alternative that can be administered on a similar scale but results in much more consistent with higher quality transfection and higher cell viability. This chapter discusses the creation and use of a simple and cost-effective device using porous membrane for performing localized electroporation.
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Acknowledgments
R. Yang acknowledges the funding from the Nebraska Center for Integrated Biomolecular Communication (NIH National Institutes of General Medical Sciences P20 GM113126), from Nebraska Center for Nanomedicine (P30 GM127200), from EPSCoR First Award, and from NSF award 1826135.
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Brooks, J., Jaberi, A., Yang, R. (2020). Microfluidic Device for Localized Electroporation. In: Li, S., Chang, L., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 2050. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9740-4_10
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DOI: https://doi.org/10.1007/978-1-4939-9740-4_10
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