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Protein–Protein Interaction Profiling in Candida albicans Revealed by Biochemical Purification–Mass Spectrometry (BP/MS)

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Yeast Systems Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2049))

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Abstract

BP/MS is a new experimental proteomic platform for systematic study of native protein complexes and global protein–protein interaction networks based on deep biochemical purification of soluble protein extracts and mass spectrometry identification of coeluting proteins. Herein, we describe the application of this methodology to draft a high-confidence protein interaction network for Candida albicans.

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Correspondence to Andrew Emili .

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Pourhaghighi, R., O’Meara, T.R., Cowen, L.E., Emili, A. (2019). Protein–Protein Interaction Profiling in Candida albicans Revealed by Biochemical Purification–Mass Spectrometry (BP/MS). In: Oliver, S.G., Castrillo, J.I. (eds) Yeast Systems Biology. Methods in Molecular Biology, vol 2049. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9736-7_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9736-7_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9735-0

  • Online ISBN: 978-1-4939-9736-7

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