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Brain Development pp 153–160Cite as

Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2047))

Abstract

Live cell imaging gives valuable insights into the dynamic biological processes within and between cells. An important aspect of live cell imaging is to keep the cells under best physiological condition and to prevent abnormal cellular behavior, which might be caused by phototoxicity during microscopy. In this chapter we describe a protocol to visualize division patterns of neural stem cells in live whole mount brains of Drosophila larvae. We also present a newly developed live cell chamber that allows us to control the environmental air during live cell imaging. The protocol can be adapted to look at a wide range of cellular and tissue behavior in the Drosophila model system.

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Acknowledgments

We thank Jean-Daniel Niederhäuser for the construction of the live cell chamber and we thank Clemens Cabernard for advising us on live cell imaging protocols.

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Authors and Affiliations

  1. Department of Biology, University of Fribourg, Fribourg, Switzerland

    Karolina Miszczak & Boris Egger

Authors
  1. Karolina Miszczak
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  2. Boris Egger
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Corresponding author

Correspondence to Boris Egger .

Editor information

Editors and Affiliations

  1. Department of Biology, University of Fribourg, Fribourg, Switzerland

    Simon G. Sprecher

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Miszczak, K., Egger, B. (2020). Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain. In: Sprecher, S. (eds) Brain Development. Methods in Molecular Biology, vol 2047. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9732-9_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9732-9_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9731-2

  • Online ISBN: 978-1-4939-9732-9

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