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Transplantation of Neural Tissue: Quail–Chick Chimeras

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Brain Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2047))

Abstract

Tissue transplantation is an important approach in developmental neurobiology to determine cell fate, to uncover inductive interactions required for tissue specification and patterning as well as to establish tissue competence and commitment. Combined with state-of-the-art molecular approaches, transplantation assays have been instrumental for the discovery of gene regulatory networks controlling cell fate choices and how such networks change over time. Avian species are among the favorite model systems for these approaches because of their accessibility and relatively large size. Here we describe two culture techniques used to generate quail–chick chimeras at different embryonic stages and methods to distinguish graft and donor tissue.

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Acknowledgments

This work is supported by the BBSRC, NIH, and ERC. We thank Anneliese Norris for assistance with New culture photography.

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Authors and Affiliations

  1. Faculty of Dental, Oral and Craniofacial Sciences, Centre for Craniofacial and Regenerative Biology, King’s College London, London, UK

    Andrea Streit

  2. Department of Cell and Developmental Biology, University College London, London, UK

    Claudio D. Stern

Authors
  1. Andrea Streit
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  2. Claudio D. Stern
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Corresponding author

Correspondence to Andrea Streit .

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Editors and Affiliations

  1. Department of Biology, University of Fribourg, Fribourg, Switzerland

    Simon G. Sprecher

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Streit, A., Stern, C.D. (2020). Transplantation of Neural Tissue: Quail–Chick Chimeras. In: Sprecher, S. (eds) Brain Development. Methods in Molecular Biology, vol 2047. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9732-9_26

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  • DOI: https://doi.org/10.1007/978-1-4939-9732-9_26

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9731-2

  • Online ISBN: 978-1-4939-9732-9

  • eBook Packages: Springer Protocols

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