Abstract
In order to differentiate T cells in vitro, co-culture systems with Notch ligand-expressing feeder cells have been in use for a long time. Here we describe a feeder-free culture condition for differentiating T cells from hematopoietic cells that are cultured on Fc-DLL4-coated plate with T-lineage cytokines. This condition is capable of efficiently differentiating hematopoietic progenitor cells (HPCs) to immature T cells expressing both CD4 and CD8. To mature those cells into functional T cells, further stimulation and culture is necessary.
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Yasui, Y., Hitoshi, Y., Kaneko, S. (2019). In Vitro Differentiation of T Cell: From Human iPS Cells in Feeder-Free Condition. In: Kaneko, S. (eds) In Vitro Differentiation of T-Cells. Methods in Molecular Biology, vol 2048. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9728-2_8
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DOI: https://doi.org/10.1007/978-1-4939-9728-2_8
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9727-5
Online ISBN: 978-1-4939-9728-2
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