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Using the Inducible Caspase-9 Suicide-Safeguard System with iPSC and Bioluminescent Tracking

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Book cover In Vitro Differentiation of T-Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2048))

Abstract

For scientists working within the field of induced pluripotent stem cells (iPSCs), this protocol will provide a thorough walk-through on how to conduct in vitro and in vivo experiments that validate the function of a particular safeguard system technology. In short, we provide instructions on how to generate inducible Caspase-9 (iC9) safeguard system with human iPSCs that act as normal or abnormal models of the cells for therapeutics to be tried after differentiation. These iC9-iPSCs should be modified prior to beginning this protocol by constitutively expressing luciferase, an enzyme capable of generating bioluminescent signals through the oxidation of the substrate luciferin. Monitoring the bioluminescent signal over time provides the information on whether a safeguard system is working or not.

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References

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Authors and Affiliations

  1. Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA

    Jonathan Villanueva, Toshinobu Nishimura & Hiromitsu Nakauchi

Authors
  1. Jonathan Villanueva
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  2. Toshinobu Nishimura
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  3. Hiromitsu Nakauchi
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Corresponding author

Correspondence to Hiromitsu Nakauchi .

Editor information

Editors and Affiliations

  1. Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Shin Kaneko

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Villanueva, J., Nishimura, T., Nakauchi, H. (2019). Using the Inducible Caspase-9 Suicide-Safeguard System with iPSC and Bioluminescent Tracking. In: Kaneko, S. (eds) In Vitro Differentiation of T-Cells. Methods in Molecular Biology, vol 2048. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9728-2_20

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  • DOI: https://doi.org/10.1007/978-1-4939-9728-2_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9727-5

  • Online ISBN: 978-1-4939-9728-2

  • eBook Packages: Springer Protocols

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