Abstract
Cell adhesion is a characteristic feature of phagocytic myeloid cells, which is important for several inflammatory processes, such as migration, invasion, and proliferation. Purinergic signaling in macrophages plays an important role in cell adhesion of this cell type to different extracellular substrates. This protocol describes the use of two different detection methods to quantify cell adhesion upon P2X7 receptor activation by extracellular ATP.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Dowling C, Kiely P (2015) Targeting protein kinase C downstream of growth factor and adhesion signalling. Cancers 7:1271–1291
Hynes RO (2002) Integrins: bidirectional, allosteric signaling machines. Cell 110:673–687
Taherian A, Li X, Liu Y, Haas TA (2011) Differences in integrin expression and signaling within human breast cancer cells. BMC Cancer 11:293
Roger S, Gillet L, Baroja-Mazo A, Surprenant A, Pelegrin P (2010) C-terminal calmodulin-binding motif differentially controls human and rat P2X7 receptor current facilitation. J Biol Chem 285:17514–17524
Yan Z, Khadra A, Li S, Tomic M, Sherman A, Stojilkovic SS (2010) Experimental characterization and mathematical modeling of P2X7 receptor channel gating. J Neurosci 30:14213–14224
Pelegrín P (2011) Many ways to dilate the P2X7 receptor pore. Br J Pharmacol 163:908–911
Roger S, Pelegrin P, Surprenant A (2008) Facilitation of P2X7 receptor currents and membrane blebbing via constitutive and dynamic calmodulin binding. J Neurosci 28:6393–6401
Furlan-freguia C, Marchese P, Gruber A, Ruggeri ZM, Ruf W (2011) P2X7 receptor signaling contributes to tissue factor-dependent thrombosis in mice. J Clin Invest 121:2932–2944
Sengstake S, Boneberg E-M, Illges H (2006) CD21 and CD62L shedding are both inducible via P2X7Rs. Int Immunol 18:1171–1178
Mishra A, Guo Y, Zhang L, More S, Weng T, Chintagari NR et al (2016) A critical role for P2X 7 receptor-induced VCAM-1 shedding and neutrophil infiltration during acute lung injury. J Immunol 197:2828–2837
Price P, Mcmillan TJ (1990) Use of the tetrazolium assay in measuring the response of human tumor cells to ionizing radiation. Cancer Res 50:1392–1396
Idziorek T, Estaquier J, De Bels F, Ameisen JC (1995) YOPRO-1 permits cytofluorometric analysis of programmed cell death (apoptosis) without interfering with cell viability. J Immunol Methods 185:249–258
Acknowledgments
This work was supported by the European Research Council (ERC-2013-CoG 614578), EU COST Program (BM1406), and Ministerio de Economia, Industria y Competitividad–Fondo Europeo de Desarrollo Regional (project no. SAF2017-88276-R).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Martínez-García, J.J., Pelegrín, P. (2020). Assessment of Cell Adhesion After Purinoceptor Activation. In: Pelegrín, P. (eds) Purinergic Signaling. Methods in Molecular Biology, vol 2041. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9717-6_27
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9717-6_27
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9716-9
Online ISBN: 978-1-4939-9717-6
eBook Packages: Springer Protocols