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Peptidomic Workflow Applied to Cerebrospinal Fluid Analysis

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Cerebrospinal Fluid (CSF) Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2044))

Abstract

Proteo-peptidomic profiling of biofluids is used to identify disease biomarkers and to study molecular mechanisms of pathology development. Previously, we studied changes in cerebrospinal fluid (CSF) and blood plasma associated with Guillain-Barre syndrome (GBS)—a rare and severe disorder of the peripheral nervous system with an unknown etiology. Here, we describe the workflow for the analysis of endogenous peptides from CSF. The procedure covers sample preparation, liquid chromatography-mass spectrometry (LC-MS) analysis, and bioinformatics analysis and allows identification of more than 1100 peptides from 181 protein groups in ~3 h from a single CSF sample derived from non-neurological, non-oncological patients.

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation

    Rustam H. Ziganshin, Sergey I. Kovalchuk & Igor V. Azarkin

Authors
  1. Rustam H. Ziganshin
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  2. Sergey I. Kovalchuk
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  3. Igor V. Azarkin
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Editor information

Editors and Affiliations

  1. Proteomics Unit, Clinical Neuroproteomics Laboratory, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, Proteored-ISCIII, Pamplona, Spain

    Enrique Santamaría

  2. Proteomics Unit, Clinical Neuroproteomics Laboratory, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, Proteored-ISCIII, Pamplona, Spain

    Joaquín Fernández-Irigoyen

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Ziganshin, R.H., Kovalchuk, S.I., Azarkin, I.V. (2019). Peptidomic Workflow Applied to Cerebrospinal Fluid Analysis. In: Santamaría, E., Fernández-Irigoyen, J. (eds) Cerebrospinal Fluid (CSF) Proteomics. Methods in Molecular Biology, vol 2044. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9706-0_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9706-0_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9705-3

  • Online ISBN: 978-1-4939-9706-0

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